Fermentation of Microalgae Biomass through Mild Acid Pretreatment for Bioethanol Production

Lee Muei Chng, Kenneth S.K. Teo, Derek Juinn Chieh Chan, Keat Teong Lee, Pey Yi Toh

Abstract


Conversion of microalgae biomass to bioethanol is actively being researched to establish a cost effective and sustainable production technology. The main challenge is to break down the carbohydrates content in the biomass to obtain fermentable sugar for subsequent fermentation process. This study focuses on the effectiveness of the usage phosphoric acid pretreatment and capability of Saccharomyces diastaticus as the fermentation microbe to produce ethanol. Scenedesmus dimorphus microalgae biomass was used as the feedstock due to its high carbohydrate content. Mild acid hydrolysis at various conditions were carried out on biomass and the hydrolysates were subjected to fermentation. The optimum condition of acid pre-treatment using phosphoric acid was determined in this study. Based on the results, bioethanol yield of 94% was obtained at optimum condition of 2.5% v/v phosphoric acid at temperature of 120 °C for 30 min. This study proved that combination of phosphoric acid pre-treatment process with Saccharomyces diastaticus yeast provides a practicable method for the production of bioethanol from microalgae.


Keywords


Mild acid treatment; Scenedesmus dimorphus; Saccharomyces diastaticus; bioethanol

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References


Asada, C., Doi, K., Sasaki, C. & Nakamura, Y. 2012. Efficient Extraction Of Starch From Microalgae Using Ultrasonic Homogenizer And Its Conversion Into Ethanol By Simultaneous Saccharification And Fermentation. Natural Resources. 3: 5.

Ghasemi, Y., Rasoul-Amini, S., Naseri, A., Montazeri-Najafabady, N., Mobasher, M. & Dabbagh, F. 2012. Microalgae Biofuel Potentials (Review). Applied Biochemistry and Microbiology. 48: 126-144.

Millati, R. 2005. Ethanol production from lignocellulosic materials: Potential of continuous cultivation, immobilisation, and Zygomycetous fungi, Chalmers University of Technology.

John, R. P., Anisha, G., Nampoothiri, K. M. & Pandey, A. 2011. Micro And Macroalgal Biomass: A Renewable Source For Bioethanol. Bioresource Technology. 102: 186-193.

Chng, L. M., Chan, D. J. & Lee, K. T. 2016. Sustainable Production Of Bioethanol Using Lipid-Extracted Biomass From Scenedesmus Dimorphus. Journal of Cleaner Production.. 130: 68-73.

Biller, P. & Ross, A. 2014. Pyrolysis GC–MS as a Novel Analysis Technique To Determine The Biochemical Composition Of Microalgae. Algal Research.. 6: 91-97.

Andersen, R. A. 2005. ‘Algal Culturing Techniques’. Academic Press.

Castro, Y. A., Ellis, J. T., Miller, C. D. & Sims, R. C. 2015. Optimization Of Wastewater Microalgae Saccharification Using Dilute Acid Hydrolysis For Acetone, Butanol, And Ethanol Fermentation. Applied Energy. 140: 14-19.

Nguyen, M. T., Choi, S. P., Lee, J., Lee, J. H. & Sim, S. J. 2009. Hydrothermal Acid Pretreatment of Chlamydomonas reinhardtii Biomass For Ethanol Production. Journal of Microbiology and Biotechnology. 19: 161-166.

López-Linares, J., Cara, C., Moya, M., Ruiz, E., Castro, E. & Romero, I. 2013. Fermentable Sugar Production From Rapeseed Straw By Dilute Phosphoric Acid Pretreatment. Industrial Crops and Products. 50: 525-531.

Moxley, G. & Zhang, Y.-H. P. 2007. More Accurate Determination Of Acid-Labile Carbohydrates In Lignocellulose By Modified Quantitative Saccharification. Energy & Fuels. 21: 3684-3688.

Silva, C., Ferreira, A., Dias, A. P. & Costa, M. 2016. A Comparison Between Microalgae Virtual Biorefinery Arrangements For Bio-Oil Production Based On Lab-Scale Results. Journal of Cleaner Production. 130: 58-67.

Simas-Rodrigues, C., Villela, H. D., Martins, A. P., Marques, L. G., Colepicolo, P. & Tonon, A. P. 2015. Microalgae for economic applications: advantages and perspectives for bioethanol. Journal of Experimental Botany. 66: 4097–4108.

Amutha, R. & Gunasekaran, P. 2001. Production of Ethanol From Liquefied Cassava Starch Using Co-Immobilized Cells of Zymomonas mobilis and Saccharomyces diastaticus. Journal of Bioscience and Bioengineering. 92: 560-564.

Pothiraj, C., Arun, A. & Eyini, M. 2015. Simultaneous Saccharification And Fermentation Of Cassava Waste For Ethanol Production. Biofuel Research Journal. 2: 196-202.

Ruiz, R. & Date, T. E. 1996. Determination of Carbohydrates In Biomass By High Performance Liquid Chromatography. Laboratory Analytical Procedure No. 002, National Renewable Research Laboratory. Citeseer.

Ehrman, T. 1996. Determination of Starch In Biomass Samples By Chemical Solubilization And Enzymatic Digestion. . Laboratory Analytical Procedure No. 016,. National Renewable Research Laboratory

Stewart, G. G. & Russell, I. 1987. Biochemistry and Genetics Of Carbohydrate Utilization By Industrial Yeast Strains. Pure and Applied Chemistry. 59: 1493-1500.

Yazdani, P., Karimi, K. & Taherzadeh, M. J. Improvement Of Enzymatic Hydrolysis Of A Marine Macro-Alga By Dilute Acid Hydrolysis Pretreatment. World Renewable Energy Congress-Sweden; 8-13 May; 2011; Linköping; Sweden, 2011. Linköping University Electronic Press, 186-191.

Gilliland, R. 1966. Saccharomyces diastaticus—a Starch‐Fermenting Yeast. Journal of the Institute of Brewing. 72: 271-275.

Harun, R. & Danquah, M. K. 2011. Influence of Acid Pre-Treatment On Microalgal Biomass For Bioethanol Production. Process Biochemistry. 46: 304-309.




DOI: https://doi.org/10.11113/jest.v1n2-2.27

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